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ecoli-protein-expression-histag-a280.md

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1# E. coli Protein Expression with His-tag Purification and Yield via A280
2
3**URL:** https://cloud.ginkgo.bio/protocols/ecoli-protein-expression-histag-a280
4**Service terms:** https://cloud.ginkgo.bio/terms/ecoli-histag-a280
5**Reviewed:** 2026-09-30; prices and turnaround below are catalog estimates.
6**Status:** Ginkgo Certified
7**Price:** $199/sample
8**Turnaround:** up to 3 weeks
9**Throughput:** Up to 96 constructs in parallel
11The catalog says up to 3 weeks, while service terms state 15-20 business days from
12DNA submission. Confirm scheduling in the accepted order.

E. coli Protein Expression with His-tag Purification and Yield via A280

URL: https://cloud.ginkgo.bio/protocols/ecoli-protein-expression-histag-a280 Service terms: https://cloud.ginkgo.bio/terms/ecoli-histag-a280 Reviewed: 2026-09-30; prices and turnaround below are catalog estimates. Status: Ginkgo Certified Price: $199/sample Turnaround: up to 3 weeks Throughput: Up to 96 constructs in parallel

The catalog says up to 3 weeks, while service terms state 15-20 business days from DNA submission. Confirm scheduling in the accepted order.

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14## Overview
16Fully automated, end-to-end service that takes DNA constructs from heat shock transformation through bacterial expression, cell lysis, magnetic His-tag (IMAC) bead purification, and quantitative yield measurement by A280 absorbance - in a single unattended workflow. Instruments include the Agilent Bravo 96/384, BioTek MultiFlo, Inheco and Cytomat incubators, and a Spark plate reader.

Overview

Fully automated, end-to-end service that takes DNA constructs from heat shock transformation through bacterial expression, cell lysis, magnetic His-tag (IMAC) bead purification, and quantitative yield measurement by A280 absorbance - in a single unattended workflow. Instruments include the Agilent Bravo 96/384, BioTek MultiFlo, Inheco and Cytomat incubators, and a Spark plate reader.

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18## Input
20- **Protein designs:** AA or DNA sequence submitted in CSV format
21- **His-tag orientation:** N-terminal or C-terminal fusion; linker sequence (default GGGS if unspecified)
22- **Known expression notes:** Disulfide bonds, cofactor requirements, toxicity concerns, or PTM needs

Input

  • Protein designs: AA or DNA sequence submitted in CSV format
  • His-tag orientation: N-terminal or C-terminal fusion; linker sequence (default GGGS if unspecified)
  • Known expression notes: Disulfide bonds, cofactor requirements, toxicity concerns, or PTM needs
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24## Output
26- **Yield Quantification:** A280 per well, converted to protein concentration (mg/mL) via the protein's molar extinction coefficient
27- **Expression Confirmation:** Fluorescence signal relative to controls
28- **OD culture growth data** reported per construct
29- **Assay Quality Metrics:** Per-run quality summary with plate-level controls
30- QC-flagged PDF results report plus downloadable raw CSV files

Output

  • Yield Quantification: A280 per well, converted to protein concentration (mg/mL) via the protein's molar extinction coefficient
  • Expression Confirmation: Fluorescence signal relative to controls
  • OD culture growth data reported per construct
  • Assay Quality Metrics: Per-run quality summary with plate-level controls
  • QC-flagged PDF results report plus downloadable raw CSV files
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32## Automated Workflow
34Four phases coordinated across automated instruments:
361. **Transformation with LB recovery** (heat shock; Inheco ATC, Agilent Bravo 384)
372. **Culture growth and harvest**
383. **Cell lysis and clarification**
394. **Magnetic bead IMAC purification with A280 yield readout** (BioTek MultiFlo, Agilent Bravo 96, Bioshake, Spark)

Automated Workflow

Four phases coordinated across automated instruments:

  1. Transformation with LB recovery (heat shock; Inheco ATC, Agilent Bravo 384)
  2. Culture growth and harvest
  3. Cell lysis and clarification
  4. Magnetic bead IMAC purification with A280 yield readout (BioTek MultiFlo, Agilent Bravo 96, Bioshake, Spark)
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41## Ordering
43- **Number of Proteins:** configurable
44- **Number of Replicates:** configurable
45- **File Upload:** CSV, Excel, FASTA, TXT, PDF, ZIP
46- **Additional Details:** free-text field for special requirements

Ordering

  • Number of Proteins: configurable
  • Number of Replicates: configurable
  • File Upload: CSV, Excel, FASTA, TXT, PDF, ZIP
  • Additional Details: free-text field for special requirements
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48## Use Cases
50- Bacterial expression and purification of soluble proteins
51- Parallel screening of up to 96 constructs for yield
52- Producing His-tagged protein for downstream assays

Use Cases

  • Bacterial expression and purification of soluble proteins
  • Parallel screening of up to 96 constructs for yield
  • Producing His-tagged protein for downstream assays
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