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echo-ms-method-onboarding.md

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1# Echo-MS Method Onboarding

3**URL:** https://cloud.ginkgo.bio/protocols/echo-ms-method-onboarding

4**Service terms:** https://cloud.ginkgo.bio/terms/echo-ms-onboarding

5**Reviewed:** 2026-09-30; prices and turnaround below are catalog estimates.

6**Status:** Ginkgo Certified

7**Price:** $799/molecule

8**Turnaround:** up to 3 weeks

Echo-MS Method Onboarding

URL: https://cloud.ginkgo.bio/protocols/echo-ms-method-onboarding Service terms: https://cloud.ginkgo.bio/terms/echo-ms-onboarding Reviewed: 2026-09-30; prices and turnaround below are catalog estimates. Status: Ginkgo Certified Price: $799/molecule Turnaround: up to 3 weeks

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10## Overview

12Echo-MS is an open-access, high-throughput mass spectrometry platform that eliminates the chromatography step. An acoustic liquid handler (Labcyte/Beckman Echo) ejects nanoliter droplets directly from a source plate into an open-port sampling interface connected to a mass spectrometer. Without a column to equilibrate, cycle times drop to 1-5 seconds per sample, enabling analysis of a full 384-well plate in ~2 hours (with replicates). Best suited for relative quantitation, screening, and titer assays where throughput matters more than chromatographic resolution.

14This protocol onboards your analyte for future experiments: MS conditions (spray voltage, curtain gas, ion source settings) and ejection parameters (volume, interval, carrier solvent) are optimized, and a simple sample-prep protocol is established. Onboard a method here before running [echo-ms-cfps-detection.md](echo-ms-cfps-detection.md).

16The terms scope this to relative quantitation in the CFPS matrix, not absolute

17quantitation or analytes requiring chromatographic separation. A calibration curve

18does not establish selectivity for unresolved interferents or a different matrix.

Overview

Echo-MS is an open-access, high-throughput mass spectrometry platform that eliminates the chromatography step. An acoustic liquid handler (Labcyte/Beckman Echo) ejects nanoliter droplets directly from a source plate into an open-port sampling interface connected to a mass spectrometer. Without a column to equilibrate, cycle times drop to 1-5 seconds per sample, enabling analysis of a full 384-well plate in ~2 hours (with replicates). Best suited for relative quantitation, screening, and titer assays where throughput matters more than chromatographic resolution.

This protocol onboards your analyte for future experiments: MS conditions (spray voltage, curtain gas, ion source settings) and ejection parameters (volume, interval, carrier solvent) are optimized, and a simple sample-prep protocol is established. Onboard a method here before running echo-ms-cfps-detection.md.

The terms scope this to relative quantitation in the CFPS matrix, not absolute quantitation or analytes requiring chromatographic separation. A calibration curve does not establish selectivity for unresolved interferents or a different matrix.

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20## Input

22(Download Template)

24- Molecule CAS ID

25- Expected reaction and conversion amount

26- Expected concentrations of substrates

27- Reaction conditions (time sensitivities, buffers, pure/lysate)

Input

(Download Template)

Molecule CAS ID
Expected reaction and conversion amount
Expected concentrations of substrates
Reaction conditions (time sensitivities, buffers, pure/lysate)

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29## Output

31For each molecule of interest:

33- Echo-MS method and calibration curve in the Cell Free Protein Expression Matrix

34- LOD + LOQ in the Cell Free Protein Expression Matrix

35- Validation run and report

36- Recommended next steps (e.g., sample-prep optimization) if method development was not successful

Output

For each molecule of interest:

Echo-MS method and calibration curve in the Cell Free Protein Expression Matrix
LOD + LOQ in the Cell Free Protein Expression Matrix
Validation run and report
Recommended next steps (e.g., sample-prep optimization) if method development was not successful

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38## Automated Workflow