material-handling.md
226 lines · skills/pylabrobot/references/material-handling.md at 5f2bc03
Most marks come from matching words in the file, so treat them as pointers.
Material handling, pumps, and environmental devices
Verified against PyLabRobot 0.2.2 on 2026-10-01. Every operation in this domain can create physical motion, pressure, heat, or stored energy. The snippets below identify APIs only; they do not connect to devices.
Raw lines 1 to 6Pumps
Stable frontend and one stable backend export:
from pylabrobot.pumps import MasterflexBackend, PumpVerified Pump methods:
run_revolutions(num_revolutions)
run_continuously(speed)
run_for_duration(speed, duration)
pump_volume(speed, volume)
halt()pump_volume() requires a PumpCalibration, converting requested volume to time or revolutions. It is an open-loop estimate, not a measured delivery. start and calibrate(duration=..., speed=..., volume=...) are not these frontend methods. stop() closes the backend; halt() requests halted flow. run_for_duration() does not halt in a finally block if its sleep is cancelled. Review interruption handling with the exact backend before live use.
MasterflexBackend(com_port) is transport-specific. Do not instantiate it during discovery. The current development machine table labels listed Masterflex L/S models and Agrowtek Pump Array Full; this is separate from release/firmware support.
Pump safety
Before a separately authorized run, verify:
- exact pump/head/tubing model, material, inner diameter, direction, occlusion, fittings, valves, clamps, and destination;
- calibrated relationship among command speed/revolutions/time and delivered volume for the current fluid, tubing age, backpressure, and temperature;
- prime/purge route, bubbles, siphoning, dead volume, residual volume, maximum pressure/flow, leak containment, and waste capacity;
- chemical/biological compatibility, cross-contamination controls, and tubing change policy;
- an accessible stop and safe behavior on disconnect, timeout, or partial delivery.
A time/speed command is not a measured volume. Record the calibration and uncertainty; use a validated scale/flow sensor if closed-loop confirmation is required.
Raw lines 36 to 54Heater shakers and shakers
Stable frontend imports:
from pylabrobot.heating_shaking import (
HamiltonHeaterShakerBackend,
HeaterShaker,
InhecoThermoshakeBackend,
)
from pylabrobot.shaking import ShakerThe stable class is InhecoThermoshakeBackend (lowercase s in Thermoshake), not the stale InhecoThermoShakeBackend.
Verified HeaterShaker methods:
set_temperature(temperature, passive=False)
get_temperature()
shake(speed, duration=None, **backend_kwargs)
stop_shaking(**backend_kwargs)
lock_plate(**backend_kwargs)
unlock_plate(**backend_kwargs)The old names set_shake_rate and set_temperature(None) are not the verified 0.2.2 frontend signatures. Use the exact device page for deactivation/cooling and do not substitute zero/None unless documented for that backend.
HeaterShaker construction requires name, dimensions, backend, and a child_location. Backend construction is also device topology-specific: HamiltonHeaterShakerBackend(index, interface) and InhecoThermoshakeBackend(index, control_box) require approved shared interfaces/controllers.
The current hosted development inventory labels Inheco Thermoshake variants WIP, Hamilton Heater Shaker and QInstruments BioShake Full, and the Opentrons Heater-Shaker Module Full. The old blanket Full claim for Inheco was stale. A 0.2.2 import does not establish current device support.
Raw lines 55 to 96Heater/shaker safety
Confirm plate compatibility, mass, balance, lid/seal, locking, condensation, spillage containment, orbit/speed limits, thermal limits, ramp/equilibration, sensor calibration, and safe unlock temperature. Never unlock or move a plate while shaking. Treat a requested setpoint as a command, not proof that the sample has reached that temperature.
Raw lines 97 to 104Temperature controllers
Stable frontend:
from pylabrobot.temperature_controlling import TemperatureControllerVerified methods:
set_temperature(temperature, passive=False)
get_temperature()
deactivate()The current development inventory includes Inheco CPAC and the Opentrons Temperature Module; these support labels do not establish 0.2.2 compatibility. Validate active cooling, condensation, plate/adapter contact, setpoint range, ramp, sensor placement, overshoot, and sample-versus-block temperature. set_temperature() does not await thermal equilibration; use wait_for_temperature(timeout=..., tolerance=...) for the block sensor. In 0.2.2 passive=True below the current temperature only skips a new setpoint command: it does not deactivate prior heating. Do not equate it with a verified safe cooling action.
Centrifuges
Stable imports:
from pylabrobot.centrifuge import Access2Backend, Centrifuge, VSpinBackendVerified frontend:
open_door()
close_door()
lock_door()
unlock_door()
spin(g, duration, **backend_kwargs)The stable method takes relative centrifugal force g, not the stale speed=... RPM argument. Converting RPM to RCF requires the correct rotor radius; never guess it.
The current development table labels both Agilent VSpin and its Access2 loader Full; older tables called VSpin Mostly. Released constructors are:
VSpinBackend(device_id=None) and Access2Backend(device_id, timeout=60) are device-specific. Do not use placeholder IDs in a live script.
The released 0.2.2 wheel includes MicroSpin(name, host, port=1000, timeout=30.0, backend=None) and pylabrobot.centrifuge.highres.MicroSpinBackend. The changelog still lists them under Unreleased, so it cannot decide release availability. The driver speaks ASCII over TCP; setup() opens a connection and stop() closes it without stopping a spinning rotor. status can block until spin-down. These contracts were source-inspected only; the review ledger records framing and limits. No factory or hardware backend was instantiated.
Centrifuge safety
Require human verification of rotor/bucket/adapter model, plate rating, orientation, balance, maximum RCF, duration, acceleration/deceleration, lid/door interlocks, loading position, clearance, maintenance, and emergency procedure. Never open/unlock while rotating or issue movement merely to test a connection. On timeout or disconnect, assume the rotor may still be moving until physically verified safe.
Raw lines 166 to 174Storage/incubation
The current development inventory includes Thermo Fisher/Heraeus Cytomat models with differing support levels and Inheco Incubator Shaker/SCILA. Their APIs are model-specific; do not use stale generic examples such as from pylabrobot.incubation import Incubator without verifying that exact symbol in the pinned wheel.
Storage moves require explicit plate identity, slot mapping, occupancy state, door/hatch/interlock state, orientation, environmental setpoints, and recovery from an interrupted handoff. Software occupancy is not physical detection.
Raw lines 175 to 186Multi-device orchestration
Do not independently gather() hardware operations just because frontends are async. Safe concurrency requires approved workcell interlocks and a scheduler that owns:
- device and plate state;
- collision zones and transfer ownership;
- door/tray/bucket/lock preconditions;
- timeouts, retries, idempotency, and partial-completion handling;
- emergency stop and restart/reconciliation behavior.
Default sequence:
- Validate manifests and transfers offline.
- Generate a non-executable simulation plan.
- Exercise software-only frontends where available.
- Review every handoff with the operator.
- Obtain explicit confirmation for the exact live protocol.
- Commission one device/move at a time under site procedures.
No-connection inspection
python3 skills/pylabrobot/scripts/inspect_backends.py \
--expected-version 0.2.2 --strictThis checks a fixed set of frontend symbols and methods without constructing devices or calling setup().
Sources
Reviewed 2026-10-01: constructors, methods and transports in the official 0.2.2 source distribution, current machine inventory, and release review. Source files inspected include pumps/pump.py, heating_shaking/heater_shaker.py, temperature_controlling/temperature_controller.py, centrifuge/centrifuge.py, and centrifuge/highres/microspin_backend.py. Physical control and transport behavior remain untested.