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ecoli-minibinder-expression-histag-a280.md

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1# E. coli Minibinder Expression with His-tag Purification and Yield via A280

3**URL:** https://cloud.ginkgo.bio/protocols/ecoli-minibinder-expression-histag-a280

4**Service terms:** https://cloud.ginkgo.bio/terms/ecoli-minibinder-histag-a280

5**Reviewed:** 2026-09-30; prices and turnaround below are catalog estimates.

6**Status:** Ginkgo Certified

7**Price:** $199/sample

8**Turnaround:** up to 3 weeks

9**Throughput:** Up to 96 constructs in parallel

E. coli Minibinder Expression with His-tag Purification and Yield via A280

URL: https://cloud.ginkgo.bio/protocols/ecoli-minibinder-expression-histag-a280 Service terms: https://cloud.ginkgo.bio/terms/ecoli-minibinder-histag-a280 Reviewed: 2026-09-30; prices and turnaround below are catalog estimates. Status: Ginkgo Certified Price: $199/sample Turnaround: up to 3 weeks Throughput: Up to 96 constructs in parallel

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11## Overview

13Fully automated, end-to-end service that takes designed minibinder candidates from heat shock transformation through bacterial expression, cell lysis, magnetic His-tag (IMAC) bead purification, and quantitative yield measurement by A280 absorbance - built to screen binder designs before scale-up. Instruments include the Agilent Bravo 96/384, BioTek MultiFlo, Inheco and Cytomat incubators, and a Spark plate reader.

Overview

Fully automated, end-to-end service that takes designed minibinder candidates from heat shock transformation through bacterial expression, cell lysis, magnetic His-tag (IMAC) bead purification, and quantitative yield measurement by A280 absorbance - built to screen binder designs before scale-up. Instruments include the Agilent Bravo 96/384, BioTek MultiFlo, Inheco and Cytomat incubators, and a Spark plate reader.

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15## Input

17- **Minibinder input:** AA or DNA sequence in CSV as specified by the intake text; the download is an E. coli `.xlsx` template. Inspect the current template and retain the requested fields when exporting.

18- **His-tag orientation:** N-terminal or C-terminal fusion; linker sequence (default GGGS if unspecified)

19- **Known expression notes:** Disulfide bonds, cofactor requirements, toxicity concerns, or PTM needs

Input

Minibinder input: AA or DNA sequence in CSV as specified by the intake text; the download is an E. coli .xlsx template. Inspect the current template and retain the requested fields when exporting.
His-tag orientation: N-terminal or C-terminal fusion; linker sequence (default GGGS if unspecified)
Known expression notes: Disulfide bonds, cofactor requirements, toxicity concerns, or PTM needs

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21## Output

23- **Yield Quantification:** A280 per well, converted to protein concentration (mg/mL) via the protein's molar extinction coefficient

24- **Expression Confirmation:** Fluorescence signal relative to controls

25- **OD culture growth data** reported per construct

26- **Assay Quality Metrics:** Per-run quality summary with plate-level controls

27- QC-flagged PDF results report plus downloadable raw CSV files

Output

Yield Quantification: A280 per well, converted to protein concentration (mg/mL) via the protein's molar extinction coefficient
Expression Confirmation: Fluorescence signal relative to controls
OD culture growth data reported per construct
Assay Quality Metrics: Per-run quality summary with plate-level controls
QC-flagged PDF results report plus downloadable raw CSV files

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29## Automated Workflow

31Four phases coordinated across automated instruments: