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ecoli-expression-purification-quantification.md

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1# E. coli Protein Expression, Purification, and Quantification
2
3**URL:** https://cloud.ginkgo.bio/protocols/ecoli-expression-purification-quantification
4**Service terms:** https://cloud.ginkgo.bio/terms/ecoli-expression-purification-quantification
5**Reviewed:** 2026-09-30; prices and turnaround below are catalog estimates.
6**Status:** Ginkgo Certified
7**Price:** $209/sample
8**Turnaround:** up to 3 weeks
9**Throughput:** Up to 96 constructs in parallel
11The catalog says up to 3 weeks, while service terms state 15-20 business days from
12DNA submission. Confirm scheduling in the accepted order.

E. coli Protein Expression, Purification, and Quantification

URL: https://cloud.ginkgo.bio/protocols/ecoli-expression-purification-quantification Service terms: https://cloud.ginkgo.bio/terms/ecoli-expression-purification-quantification Reviewed: 2026-09-30; prices and turnaround below are catalog estimates. Status: Ginkgo Certified Price: $209/sample Turnaround: up to 3 weeks Throughput: Up to 96 constructs in parallel

The catalog says up to 3 weeks, while service terms state 15-20 business days from DNA submission. Confirm scheduling in the accepted order.

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14## Overview
16Fully automated, end-to-end service that takes DNA constructs from heat shock transformation through bacterial expression, cell lysis, magnetic His-tag (IMAC) bead purification, quantitative yield measurement by A280 absorbance, and LabChip-based purity and size assessment - all in a single unattended workflow. Instruments include the Agilent Bravo 96/384, BioTek MultiFlo, Inheco and Cytomat incubators, a Spark plate reader, and the Revvity LabChip.

Overview

Fully automated, end-to-end service that takes DNA constructs from heat shock transformation through bacterial expression, cell lysis, magnetic His-tag (IMAC) bead purification, quantitative yield measurement by A280 absorbance, and LabChip-based purity and size assessment - all in a single unattended workflow. Instruments include the Agilent Bravo 96/384, BioTek MultiFlo, Inheco and Cytomat incubators, a Spark plate reader, and the Revvity LabChip.

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18## Input
20- **Protein designs:** AA or DNA sequence submitted in CSV format
21- **His-tag orientation:** N-terminal or C-terminal fusion; linker sequence (default GGGS if unspecified)
22- **Known expression notes:** Disulfide bonds, cofactor requirements, toxicity concerns, or PTM needs

Input

  • Protein designs: AA or DNA sequence submitted in CSV format
  • His-tag orientation: N-terminal or C-terminal fusion; linker sequence (default GGGS if unspecified)
  • Known expression notes: Disulfide bonds, cofactor requirements, toxicity concerns, or PTM needs
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24## Output
26- **Yield Quantification:** A280 per well, converted to protein concentration (mg/mL) via the protein's molar extinction coefficient
27- **Expression Confirmation:** Fluorescence signal relative to controls
28- **Purity & Size Assessment:** LabChip-based purity percentage and apparent molecular weight per construct, with virtual gel images
29- **OD culture growth data** reported per construct
30- **Assay Quality Metrics:** Per-run quality summary with plate-level controls
31- QC-flagged PDF results report plus downloadable raw CSV files

Output

  • Yield Quantification: A280 per well, converted to protein concentration (mg/mL) via the protein's molar extinction coefficient
  • Expression Confirmation: Fluorescence signal relative to controls
  • Purity & Size Assessment: LabChip-based purity percentage and apparent molecular weight per construct, with virtual gel images
  • OD culture growth data reported per construct
  • Assay Quality Metrics: Per-run quality summary with plate-level controls
  • QC-flagged PDF results report plus downloadable raw CSV files
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33## Automated Workflow
35Five phases coordinated across automated instruments:
371. **Transformation with LB recovery** (heat shock)
382. **Culture growth and harvest**
393. **Cell lysis**
404. **Magnetic bead IMAC purification with A280 yield readout** (BioTek MultiFlo, Agilent Bravo 96, Bioshake, Spark)
415. **LabChip purity / size assessment** (Revvity LabChip)

Automated Workflow

Five phases coordinated across automated instruments:

  1. Transformation with LB recovery (heat shock)
  2. Culture growth and harvest
  3. Cell lysis
  4. Magnetic bead IMAC purification with A280 yield readout (BioTek MultiFlo, Agilent Bravo 96, Bioshake, Spark)
  5. LabChip purity / size assessment (Revvity LabChip)
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43## Ordering
45- **Number of Proteins:** configurable
46- **Number of Replicates:** configurable
47- **File Upload:** CSV, Excel, FASTA, TXT, PDF, ZIP
48- **Additional Details:** free-text field for special requirements

Ordering

  • Number of Proteins: configurable
  • Number of Replicates: configurable
  • File Upload: CSV, Excel, FASTA, TXT, PDF, ZIP
  • Additional Details: free-text field for special requirements
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50## Use Cases
52- Bacterial expression with purified yield plus purity/size profiling in one run
53- Characterizing up to 96 constructs before scale-up
54- Producing and QC-ing His-tagged protein for downstream work

Use Cases

  • Bacterial expression with purified yield plus purity/size profiling in one run
  • Characterizing up to 96 constructs before scale-up
  • Producing and QC-ing His-tagged protein for downstream work
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