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cell-free-protein-expression-validation.md

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1# Cell Free Protein Expression Validation
2
3**URL:** https://cloud.ginkgo.bio/protocols/cell-free-protein-expression-validation
4**Service terms:** https://cloud.ginkgo.bio/terms/cfps-validation
5**Reviewed:** 2026-09-30; prices and turnaround below are catalog estimates.
6**Status:** Ginkgo Certified
7**Price:** $39/sample
8**Turnaround:** up to 10 days
9

Cell Free Protein Expression Validation

URL: https://cloud.ginkgo.bio/protocols/cell-free-protein-expression-validation Service terms: https://cloud.ginkgo.bio/terms/cfps-validation Reviewed: 2026-09-30; prices and turnaround below are catalog estimates. Status: Ginkgo Certified Price: $39/sample Turnaround: up to 10 days

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10## Overview
12Fastest path from a protein sequence to a quantitative go/no-go readout on expression. Uses a proprietary reconstituted E. coli transcription-translation (cell-free protein synthesis, CFPS) system. Reactions complete in 4-16 hours. Designed for early-stage screening, novel construct evaluation, and rapid triage of candidate sequences before committing resources to downstream optimization or purification.

Overview

Fastest path from a protein sequence to a quantitative go/no-go readout on expression. Uses a proprietary reconstituted E. coli transcription-translation (cell-free protein synthesis, CFPS) system. Reactions complete in 4-16 hours. Designed for early-stage screening, novel construct evaluation, and rapid triage of candidate sequences before committing resources to downstream optimization or purification.

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14## Input
16- **Sequence:** The protocol page currently links a CFPS `.xlsx` template for linear DNA; service terms request protein or DNA in FASTA. Download the current template and resolve the format with Ginkgo before submission.
17- Sequences up to 1800 bp supported
18- Specify replicates, linear DNA versus clonal plasmid synthesis, and known expression constraints.

Input

  • Sequence: The protocol page currently links a CFPS .xlsx template for linear DNA; service terms request protein or DNA in FASTA. Download the current template and resolve the format with Ginkgo before submission.
  • Sequences up to 1800 bp supported
  • Specify replicates, linear DNA versus clonal plasmid synthesis, and known expression constraints.
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20## Output
22- **Expression Confirmation:** Verification of target protein at expected molecular weight
23- **Baseline Titer:** Initial quantitative yield measurement (mg/L)
24- **Initial Purity:** Percentage of target protein vs. impurities, delivered with virtual gel images

Output

  • Expression Confirmation: Verification of target protein at expected molecular weight
  • Baseline Titer: Initial quantitative yield measurement (mg/L)
  • Initial Purity: Percentage of target protein vs. impurities, delivered with virtual gel images
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26## Automated Workflow

Automated Workflow

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28### Phase 1 - CFPS Reaction Setup & Incubation
301. Retrieve plates
312. Stamp DNA templates
323. Seal plate
334. Incubate shaking at 30 deg C

Phase 1 - CFPS Reaction Setup & Incubation

  1. Retrieve plates
  2. Stamp DNA templates
  3. Seal plate
  4. Incubate shaking at 30 deg C
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35### Phase 2 - Quantification Prep
371. Dispense PBS diluent
382. Seal plate
393. Store at 4 deg C

Phase 2 - Quantification Prep

  1. Dispense PBS diluent
  2. Seal plate
  3. Store at 4 deg C
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41### Phase 3 - LabChip Quantification
431. Unseal plate
442. LabChip quantification
453. Seal plate
464. Store at 4 deg C

Phase 3 - LabChip Quantification

  1. Unseal plate
  2. LabChip quantification
  3. Seal plate
  4. Store at 4 deg C
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48## Protocol Parameters
50- Payloads & Reagents
51- Bravo Stamp
52- HiG Centrifuge
53- Incubation & Storage

Protocol Parameters

  • Payloads & Reagents
  • Bravo Stamp
  • HiG Centrifuge
  • Incubation & Storage
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55## Ordering
57- **Number of Proteins:** configurable
58- **Number of Replicates:** configurable
59- **File Upload:** CSV, Excel, FASTA, TXT, PDF, ZIP
60- **Additional Details:** free-text field for special requirements

Ordering

  • Number of Proteins: configurable
  • Number of Replicates: configurable
  • File Upload: CSV, Excel, FASTA, TXT, PDF, ZIP
  • Additional Details: free-text field for special requirements
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62## Use Cases
64- Screening candidate protein sequences for expressibility
65- Go/no-go decisions before investing in optimization
66- Evaluating novel constructs in a cell-free system
67- Comparing expression levels across sequence variants

Use Cases

  • Screening candidate protein sequences for expressibility
  • Go/no-go decisions before investing in optimization
  • Evaluating novel constructs in a cell-free system
  • Comparing expression levels across sequence variants
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