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cfps-strep-tag-purification-a280.md

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1# Cell Free Protein Expression with Strep-tag Purification and Yield via A280

3**URL:** https://cloud.ginkgo.bio/protocols/cfps-strep-tag-purification-a280

4**Service terms:** https://cloud.ginkgo.bio/terms/cfps-strep-tag-a280

5**Reviewed:** 2026-09-30; prices and turnaround below are catalog estimates.

6**Status:** Ginkgo Certified

7**Price:** $149/sample

8**Turnaround:** up to 11 days

9**Throughput:** Up to 88 constructs per run (1 column reserved for controls), 96-well format

Cell Free Protein Expression with Strep-tag Purification and Yield via A280

URL: https://cloud.ginkgo.bio/protocols/cfps-strep-tag-purification-a280 Service terms: https://cloud.ginkgo.bio/terms/cfps-strep-tag-a280 Reviewed: 2026-09-30; prices and turnaround below are catalog estimates. Status: Ginkgo Certified Price: $149/sample Turnaround: up to 11 days Throughput: Up to 88 constructs per run (1 column reserved for controls), 96-well format

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11## Overview

13End-to-end automated expression and purification of StrepII-tagged proteins in 100 uL CFPS reactions, followed by StreptactinXT magnetic bead purification and A280 quantification. The catalog mentions 20-hour expression and LabChip, but the service terms describe typical 4-16-hour reactions and promise A280 yield/reporting without a LabChip deliverable. Confirm incubation and readout scope in the order; choose the [A280 + LabChip tier](cfps-expression-purification-quantification.md) when purity/size data are required.

Overview

End-to-end automated expression and purification of StrepII-tagged proteins in 100 uL CFPS reactions, followed by StreptactinXT magnetic bead purification and A280 quantification. The catalog mentions 20-hour expression and LabChip, but the service terms describe typical 4-16-hour reactions and promise A280 yield/reporting without a LabChip deliverable. Confirm incubation and readout scope in the order; choose the A280 + LabChip tier when purity/size data are required.

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15## Input

17- **DNA Input:** Linear DNA sequence

18- **Tag Orientation:** N-terminal or C-terminal Strep-II tag fusion (validated with C-terminal tags; success is protein-dependent)

19- **Format:** Up to 88 constructs per run, 1 column of wells for controls

Input

DNA Input: Linear DNA sequence
Tag Orientation: N-terminal or C-terminal Strep-II tag fusion (validated with C-terminal tags; success is protein-dependent)
Format: Up to 88 constructs per run, 1 column of wells for controls

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21## Output

23- **Yield Quantification:** A280 per well, converted to protein concentration (mg/mL) via the protein's molar extinction coefficient

24- **Expression Confirmation:** Fluorescence signal relative to controls

25- **Assay Quality Metrics:** Per-run quality summary with plate-level controls

Output

Yield Quantification: A280 per well, converted to protein concentration (mg/mL) via the protein's molar extinction coefficient
Expression Confirmation: Fluorescence signal relative to controls
Assay Quality Metrics: Per-run quality summary with plate-level controls

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27## Automated Workflow