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pichia-protein-expression-labchip.md

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1# Pichia Protein Expression Validation (LabChip)
2
3**URL:** https://cloud.ginkgo.bio/protocols/pichia-protein-expression-labchip
4**Service terms:** https://cloud.ginkgo.bio/terms/pichia-protein-expression-validation
5**Reviewed:** 2026-09-30; prices and turnaround below are catalog estimates.
6**Status:** Ginkgo Certified
7**Price:** $89/sample
8**Turnaround:** up to 4 weeks
9**Throughput:** Up to 96 constructs per run

Pichia Protein Expression Validation (LabChip)

URL: https://cloud.ginkgo.bio/protocols/pichia-protein-expression-labchip Service terms: https://cloud.ginkgo.bio/terms/pichia-protein-expression-validation Reviewed: 2026-09-30; prices and turnaround below are catalog estimates. Status: Ginkgo Certified Price: $89/sample Turnaround: up to 4 weeks Throughput: Up to 96 constructs per run

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11## Overview
13End-to-end automated workflow using Ginkgo's proprietary *Pichia pastoris* strains to express and characterize secreted proteins. Strains progress through three sequential preculture and production fermentation stages (multi-stage fed-batch-style growth). Secreted protein expression is quantified by size and purity via microfluidic capillary electrophoresis (LabChip). Useful for targets that express better in a eukaryotic secretion system than in E. coli or cell-free.

Overview

End-to-end automated workflow using Ginkgo's proprietary Pichia pastoris strains to express and characterize secreted proteins. Strains progress through three sequential preculture and production fermentation stages (multi-stage fed-batch-style growth). Secreted protein expression is quantified by size and purity via microfluidic capillary electrophoresis (LabChip). Useful for targets that express better in a eukaryotic secretion system than in E. coli or cell-free.

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15## Input
17- **Sequence input:** AA or DNA in CSV per service terms, with known expression constraints. The page labels its template `.xlsx`, but its download URL ends in `.csv`; inspect the downloaded format rather than renaming the extension.

Input

  • Sequence input: AA or DNA in CSV per service terms, with known expression constraints. The page labels its template .xlsx, but its download URL ends in .csv; inspect the downloaded format rather than renaming the extension.
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19## Output
21- **Expression Confirmation:** Verification of the target protein at the expected molecular weight
22- **Yield estimate:** Reference-standard estimate (ug/mL) above the detection limit; absolute yield is not guaranteed
23- **Initial Purity:** Percentage of target protein vs. impurities, delivered with virtual gel images
24- Results reported as protein size (kDa), purity (%), and relative concentration, with growth/QC context; use these for comparative expression ranking

Output

  • Expression Confirmation: Verification of the target protein at the expected molecular weight
  • Yield estimate: Reference-standard estimate (ug/mL) above the detection limit; absolute yield is not guaranteed
  • Initial Purity: Percentage of target protein vs. impurities, delivered with virtual gel images
  • Results reported as protein size (kDa), purity (%), and relative concentration, with growth/QC context; use these for comparative expression ranking
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26## Automated Workflow
281. **Transformation** using 384-well plates
292. **Multi-stage fermentation** (three sequential preculture and production stages, fed-batch-style growth)
303. **Supernatant harvest** by high-speed centrifugation
314. **Quantification Prep** - dispense PBS diluent (BioTek MultiFlo), seal (Agilent PlateLoc), store at 4 deg C (HRB TundraStore)
325. **LabChip protein quantification** (size, purity, relative concentration)

Automated Workflow

  1. Transformation using 384-well plates
  2. Multi-stage fermentation (three sequential preculture and production stages, fed-batch-style growth)
  3. Supernatant harvest by high-speed centrifugation
  4. Quantification Prep - dispense PBS diluent (BioTek MultiFlo), seal (Agilent PlateLoc), store at 4 deg C (HRB TundraStore)
  5. LabChip protein quantification (size, purity, relative concentration)
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34## Ordering
36- **Number of Proteins:** configurable
37- **Number of Replicates:** configurable
38- **File Upload:** CSV, Excel, FASTA, TXT, PDF, ZIP
39- **Additional Details:** free-text field for special requirements

Ordering

  • Number of Proteins: configurable
  • Number of Replicates: configurable
  • File Upload: CSV, Excel, FASTA, TXT, PDF, ZIP
  • Additional Details: free-text field for special requirements
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41## Use Cases
43- Expressing secreted/eukaryotic proteins that are difficult in E. coli or cell-free
44- Validating expressibility in *Pichia pastoris* before scale-up
45- Size/purity profiling of secreted targets

Use Cases

  • Expressing secreted/eukaryotic proteins that are difficult in E. coli or cell-free
  • Validating expressibility in Pichia pastoris before scale-up
  • Size/purity profiling of secreted targets
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